Question: using beadarray to process iscan expression data - error using readIllumina()
gravatar for Darren Plant
5.0 years ago by
Darren Plant70
Darren Plant70 wrote:
Dear All, I'm hoping to use beadarray to process illuminaHT-12 v4 expression data (just one chip) that was generated using iScan. I have .locs files and .tif files for both swath1 and swath2 and have used processSwathData() to split the .txt file appropriately. I'm now struggling to execute the readIllumina() function for some reason (below). Do i need to modify my sampleSheet following processSwathData() to take into account the fact that I now have two .txt files for each array? I can't get passed the error message below. > processSwathData(inputDir = "data/9259561003", outputDir = "data/9259561003", twoColour=NULL, textstring="_perBeadFile.txt", segmentHeight=326, segmentWidth=397, fullOutput=TRUE, newTextString=".txt") 9259561003_A 9259561003_B 9259561003_C 9259561003_D 9259561003_E 9259561003_F 9259561003_G 9259561003_H 9259561003_I 9259561003_J 9259561003_K 9259561003_L > sampleSheetFile <- paste("data/9259561003", "/sampleSheet.csv", sep = "") > readLines(sampleSheetFile) [1] "[Header],,," "Investigator Name,Darren,," "Project Name,RMS Whole Genome Expression Profiling,," [4] "Experiment Name,Chip 1,," "Date,31/05/2013,," "[Data],,," [7] "Sample_Name,Sample_Group,Sentrix_ID,Sentrix_Position" "RMS06012,poor,9259561003,A" "RMS12038,good,9259561003,B" [10] "RMS12001,good,9259561003,C" "RMS21004,poor,9259561003,D" "RMS12039,poor,9259561003,E" [13] "RMS12016,good,9259561003,F" "RMS12032,good,9259561003,G" "RMS12041,poor,9259561003,H" [16] "RMS15003,poor,9259561003,I" "RMS20020,good,9259561003,J" "RMS20022,good,9259561003,K" [19] "RMS10025,poor,9259561003,L" > data <- readIllumina("data/9259561003", sampleSheet = sampleSheetFile, useImages = TRUE, illuminaAnnotation = "Humanv4") Sample Sheet D:\work\RMS\data\9259561003\sampleSheet.csv will be used to read the data Error in analyseDirectory(dir = x, sectionNames = as.character(dirs[[x]]), : Directory does not exist. Thanks in advance! Best wishes, Darren [[alternative HTML version deleted]]
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