limmaGUI and Agilent output files!
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Adrian Peres ▴ 30
@adrian-peres-861
Last seen 7.3 years ago
Hi everyone Is anybody out there using limmaGUI for microarray analysis? I have Agilent output .txt files and I wonder if they can be imported in limmaGUI. I have tried but it doesn't work! Can anyone give a hint on how to start...? This program is totally new for me and using the "Help" I could not figure out! Thank, Adrian confidentiality notice: The information contained in this e-mail is confidential and...{{dropped}}
Microarray limmaGUI Microarray limmaGUI • 602 views
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@james-wettenhall-153
Last seen 7.3 years ago
Hi Adrian, > I have tried but it doesn't work! You need to describe the error in more detail than that for us to be able to help you. limmaGUI does support Agilent files. (Just follow the instructions in the Swirl example, and replace .spot files with your Agilent files. If you get any errors when you try to read the Agilent files into limmaGUI, please report them as accurately as possible on this mailing list (including errors in message boxes and in the main R console). Then if you have any trouble, we can work out whether the problem is in limma (the underlying microarray data analyser) or the GUI (Graphical User Interface). You should also tell us the version number of limma and limmaGUI you are using and if possible, send the header of one of your Agilent files (i.e. the first few lines, before the main table of raw data) and perhaps a few rows of the actual raw data. Regards, James
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