Question: ddCt calculation and statistics

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Guest User •

**12k**wrote:
Hi all,
my name is Luca Peruzza, I am a student working at the University of
Trieste.
I approached R software because I have to calculate Gene Expression
and I found this R library ("ddCt") that is exactly what i need
because I need to calculate the Gene Expression, the propagation of
the error, and I want to perform statistical analysis with my data.
In the laboratory we have a Biorad RT-PCR. I can export the file
generated from the Biorad software onto Exel.
I'm new to R, but I tried to use the package ddCt with my chief (who
knows R) and we read all the documentation about the package, but we
don't understand how to use it. In particular we can't understand the
following:
- how has to be the file that you load?
- how can the package understand which gene is the reference, which
one is the target, ....
- how can you perform a t-test using the data?
I can provide an example of the output of the qPCR, if needed.
Can someone be so kind to explain how to use it?
Would be the best if you could provide us a script with the commands
and explanations of them!
Thanking you in advance for the time you spent with me, I want to wish
you a pleasant journey.
-- output of sessionInfo():
load.path = "."
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