yes Gregory.. On Tue, Oct 1, 2013 at 2:36 PM, gregory voisin <voisingreg@yahoo.fr> wrote: > Hi if I understand well: you have 1 cel for the control and 1 cel for the > experiment. > You have no replication? > > > > > ------------------------------ > *De :* deepika lakhwani <lakhwanideepika@gmail.com> > *À :* bioconductor@r-project.org > *Envoyé le :* Mardi 1 octobre 2013 19h27 > *Objet :* [BioC] Fwd: Regarding miRNA analysis > > hello... > > Can anyone clear one more thing about the miRNA analysis. As I mention > earlier I have two miRNA cel files(rice) for one is control and other one > is experiment . when I did analysis for differential miRNA using affylmgUI > package. I got total 20707 mirna with significant and non significant P > value. > In this analysis, how can we identify that out of 20707 miRNA, which miRNA > is hybridize properly. > > > > thanks > > regards > deepika > > > ---------- Forwarded message ---------- > From: deepika lakhwani <lakhwanideepika@gmail.com> > Date: Tue, Oct 1, 2013 at 10:12 AM > Subject: Regarding miRNA analysis > To: bioconductor@r-project.org > > > hello.. > > I have miRNA hybridized CEL files for control and experiment. now i want to > find out differential miRNA and also want to identify that how many miRNAs > are hybridized. I do not know which package and method was used for miRNA > analysis. > > I did Microarray analysis of RNA cel files and for that i used affylmGUI > package and RMA method. but for the differential miRNA analysis same > package can we used? If yes then i did the analysis and i got 20707 miRNAs > with M and P values. Now i did not get that how many miRNAs are hybridized. > > I am so confused so Can any one help me in the field of micro array > analysis? > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > [[alternative HTML version deleted]]