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@isha-pandey-phd-scholar-bits-pilani-campus-6195

Last seen 8.0 years ago

Hi
I am analyzing microarray data *of Plasmodium falciparum* parasite ,
un
like the normal CGH, we have CY3 labeled complicated malaria vs. CY5
labeled Uncomplicated malaria so here ratio is between uncomplicated
vs.
complicated i.e., CY3/CY5, but I also want to know what is the copy
number
variation in CY3 labeled sample and cy5 labeled sample individually
because both of them are disease conditions, so is there any way to
analyze
this data,
Steps which I am following is:
· I have taken g mean intensity of probes labeled with cy3 and
cy5
· Taken a standard deviation of background mean intensities,
then
multiplied this mean intensity with 2.6,
· After multiplying added with bg mean intensity and then
subtracted with the gmean intensity
· Is this the right manner of anlaysing this data
· Or is there any other way out, since I am new to this I need
to
know in which way should I proceed please help me out
Array platform is Agilent array 44k and for only single
array, and please let me know is there any way to find out how many
fold
the copy number variation is taking place
Regards
Isha Pandey
Email- isha.pandey@bits-pilani.ac.in, aahana10123@gmail.com
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