Question: Questions about input data with limma GUI
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gravatar for hélène régé
15.2 years ago by
hélène régé10 wrote:
Dear all, Hello, I'm a student, and I want to analyse my microarray data with limma GUI but the program bug probably because my data isn't in a good format. My data comes from the software Array-Pro Analyser with a clone number, raw intensity of cells A labeled with Cy3, raw intensity of cells B labeled with Cy5 and the same thing for the dye-swap. I haven't background data (the correction was done by Array-Pro). For input my data in limma GUI, I create this 2 text files : > > SpotType ID Name Color > > cDNA * * black > > Alien1 Alien1 * blue > > Alien2 Alien2 * blue > > Plant Plant * blue > > plant plant * blue > > GFP GFP * red > > > > > > SlideNumber FileNameCy3 FileNameCy5 Cy3 Cy5 > > 1 lame1w647.txt lame1w555.txt Ooc Cs > > 2 lame2w647.txt lame2w555.txt Cs Oos > > > > the text file like lame1w647.txt have 2 columns, the first contains the > > clone number and the second the raw intensity > > > > > > > > when I try to load myTarget.txt, this error messages appear : > > Error in scan(fili=......, : line 1 did not have 6 elements > > Erro in colnames(Targets) : Objest "Targets" not found > > Error in assign("Targets"..... > > I want just analyse and no visualize and so i don't want use Spot format. > > Please help me for resolve this probleme > > > > Thank you very much > > > > H?l?ne
microarray gui limma • 445 views
ADD COMMENTlink written 15.2 years ago by hélène régé10
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