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hélène régé
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@helene-rege-874
Last seen 9.6 years ago
Dear all,
Hello,
I'm a student, and I want to analyse my microarray data with limma GUI
but
the program bug probably because my data isn't in a good format. My
data
comes from the software Array-Pro Analyser with a clone number, raw
intensity of cells A labeled with Cy3, raw intensity of cells B
labeled with
Cy5 and the same thing for the dye-swap. I haven't background data
(the
correction was done by Array-Pro).
For input my data in limma GUI, I create this 2 text files :
> > SpotType ID Name Color
> > cDNA * * black
> > Alien1 Alien1 * blue
> > Alien2 Alien2 * blue
> > Plant Plant * blue
> > plant plant * blue
> > GFP GFP * red
> >
> >
> > SlideNumber FileNameCy3 FileNameCy5 Cy3 Cy5
> > 1 lame1w647.txt lame1w555.txt Ooc Cs
> > 2 lame2w647.txt lame2w555.txt Cs Oos
> >
> > the text file like lame1w647.txt have 2 columns, the first
contains the
> > clone number and the second the raw intensity
> >
> >
> >
> > when I try to load myTarget.txt, this error messages appear :
> > Error in scan(fili=......, : line 1 did not have 6 elements
> > Erro in colnames(Targets) : Objest "Targets" not found
> > Error in assign("Targets".....
> >
I want just analyse and no visualize and so i don't want use Spot
format.
> > Please help me for resolve this probleme
> >
> > Thank you very much
> >
> > H?l?ne