Question: EximiR - Limma - miRCURY LNA - Exiqon
0
gravatar for Paolo Kunderfranco
5.5 years ago by
Paolo Kunderfranco350 wrote:
Dear All, I a new in this kind of analyisis. I have to analyze some old data obtained with the technology: miRCURY LNA¬ô from Exiqon. I use routinely limma and lumi package to anlayze Illumina BeadChip microarray but is the first attempt to analyze data obtained form this platform. I used ExiMiR package for normalizing miRNA expression data and obtained an ExpressionSet object. > eset.spike ExpressionSet (storageMode: lockedEnvironment) assayData: 1988 features, 8 samples element names: exprs, se.exprs protocolData: none phenoData sampleNames: 1_Exiqon_14076908_S01_Cropped 1_Exiqon_14076910_S01_Cropped ... 0_Exiqon_14076913_S01_Cropped (8 total) varLabels: sample varMetadata: labelDescription featureData: none experimentData: use 'experimentData(object)' Annotation: galenv.imagene.20140108105629478 I have 4 samples, 2 are control and 2 are cancer patients (Hy3 and Hy5) What I would like to do now is to use limma package to assess for differential expression between the two groups but I am really confused about dual color microarray and I can't proceed further without any hint, Could You please help me? Many Thanks, Paolo [[alternative HTML version deleted]]
ADD COMMENTlink written 5.5 years ago by Paolo Kunderfranco350
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 363 users visited in the last hour