EximiR - Limma - miRCURY LNA - Exiqon
0
0
Entering edit mode
@paolo-kunderfranco-5158
Last seen 4.3 years ago
Dear All, I a new in this kind of analyisis. I have to analyze some old data obtained with the technology: miRCURY LNA¬ô from Exiqon. I use routinely limma and lumi package to anlayze Illumina BeadChip microarray but is the first attempt to analyze data obtained form this platform. I used ExiMiR package for normalizing miRNA expression data and obtained an ExpressionSet object. > eset.spike ExpressionSet (storageMode: lockedEnvironment) assayData: 1988 features, 8 samples element names: exprs, se.exprs protocolData: none phenoData sampleNames: 1_Exiqon_14076908_S01_Cropped 1_Exiqon_14076910_S01_Cropped ... 0_Exiqon_14076913_S01_Cropped (8 total) varLabels: sample varMetadata: labelDescription featureData: none experimentData: use 'experimentData(object)' Annotation: galenv.imagene.20140108105629478 I have 4 samples, 2 are control and 2 are cancer patients (Hy3 and Hy5) What I would like to do now is to use limma package to assess for differential expression between the two groups but I am really confused about dual color microarray and I can't proceed further without any hint, Could You please help me? Many Thanks, Paolo [[alternative HTML version deleted]]
miRNA Microarray Cancer limma lumi ExiMiR miRNA Microarray Cancer limma lumi ExiMiR • 1.1k views
ADD COMMENT

Login before adding your answer.

Traffic: 357 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6