problem in getVarianceStabilizedData
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@suparna-mitra-6359
Last seen 9.6 years ago
Hi All, I am having a problem while running getVarianceStabilizedData in DDSeq2 package. data.vsd<-getVarianceStabilizedData(data) Error in (function (classes, fdef, mtable) : unable to find an inherited method for function ‘dispersionFunction’ for signature ‘"CountDataSet"’ Though the function looks okay > dispersionFunction standardGeneric for "dispersionFunction" defined from package "DESeq2" function (object) standardGeneric("dispersionFunction") <environment: 0x7fe7a9c5d140=""> Methods may be defined for arguments: object Use showMethods("dispersionFunction") for currently available ones. Can anybody please help? Thanks, Mitra. [[alternative HTML version deleted]]
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@mikelove
Last seen 13 hours ago
United States
hi Suparna, CountDataSet is the class used in DESeq, while DESeqDataSet is the class used in DESeq2. You can convert a CountDataSet to a DESeqDataSet using the steps outlined in the vignette, "1.2.3 Count matrix input". Mike On Sun, Jan 26, 2014 at 11:28 PM, Suparna Mitra <suparna.mitra.sm@gmail.com>wrote: > Hi All, > I am having a problem while running getVarianceStabilizedData in DDSeq2 > package. > > data.vsd<-getVarianceStabilizedData(data) > Error in (function (classes, fdef, mtable) : > unable to find an inherited method for function ‘dispersionFunction’ for > signature ‘"CountDataSet"’ > > Though the function looks okay > > dispersionFunction > standardGeneric for "dispersionFunction" defined from package "DESeq2" > > function (object) > standardGeneric("dispersionFunction") > <environment: 0x7fe7a9c5d140=""> > Methods may be defined for arguments: object > Use showMethods("dispersionFunction") for currently available ones. > > Can anybody please help? > Thanks, > Mitra. > > [[alternative HTML version deleted]] > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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Thanks a lot Michael, Thats a great help. Best wishes, Suparna Dr. Suparna Mitra Department of Molecular and Clinical Pharmacology Institute of Translational Medicine University of Liverpool Block A: Waterhouse Buildings 1-5 Brownlow Street Liverpool L69 3GL Tel. +44 (0)151 795 5414 M: +44 (0) 7523228621 Internal ext: 55401 On 27 January 2014 21:18, Michael Love <michaelisaiahlove@gmail.com> wrote: > hi Suparna, > > CountDataSet is the class used in DESeq, while DESeqDataSet is the class > used in DESeq2. > > You can convert a CountDataSet to a DESeqDataSet using the steps outlined > in the vignette, "1.2.3 Count matrix input". > > Mike > > > > > On Sun, Jan 26, 2014 at 11:28 PM, Suparna Mitra < > suparna.mitra.sm@gmail.com> wrote: > >> Hi All, >> I am having a problem while running getVarianceStabilizedData in DDSeq2 >> package. >> >> data.vsd<-getVarianceStabilizedData(data) >> Error in (function (classes, fdef, mtable) : >> unable to find an inherited method for function ‘dispersionFunction’ for >> signature ‘"CountDataSet"’ >> >> Though the function looks okay >> > dispersionFunction >> standardGeneric for "dispersionFunction" defined from package "DESeq2" >> >> function (object) >> standardGeneric("dispersionFunction") >> <environment: 0x7fe7a9c5d140=""> >> Methods may be defined for arguments: object >> Use showMethods("dispersionFunction") for currently available ones. >> >> Can anybody please help? >> Thanks, >> Mitra. >> >> [[alternative HTML version deleted]] >> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > [[alternative HTML version deleted]]
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