CHARM qcReport Question
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@zeynep-ozkeserli-5250
Last seen 9.3 years ago
Turkey
Hi all (and if any chance, Dr. Aryee), I am working with 112 (56 532, 56 635) NimbleGen Promoter Medip arrays to perform a CHARM analysis. First of all, I wanted to obtain the qc values and have a qcReport but I get this error message: Error in plot.window(...) : need finite 'xlim' values My code is: dataDir<- getwd() pd<- readDelim( files=pd$fileName, path=dataDir, sampleKey=pd) rawData<- readCharm(files=pd$fileName, path=dataDir, sampleKey=pd) qual<- qcReport(rawData, file="qcReport.pdf") R version: 3.03 Memory: 47.2 GiB Processor: Intel Xeon CPU E5645 @ 2.40GHz x 19 OS type: 64-bit Disk. 146.2 GB Has anyone have any idea about this? Thank you in advance, Best, Zeynep [[alternative HTML version deleted]]
charm charm • 947 views
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Entering edit mode
@zeynep-ozkeserli-5250
Last seen 9.3 years ago
Turkey
Hi All, I think I have to add these too to my previous message about qcReport error. After getting the error, I tried to go on and try next steps. I was able to get the control Indexes but again got an error message at controlQC step: cqc<- controlQC(rawData=rawData, controlIndex= ctrlIdx, IDcol="sampleID", expcol="tissue", ylimits=c(-6,8), outfile= "boxplots_check.pdf", height=7, width=9) Spatial normalization Background removal Error in density.default(x, kernel = "epanechnikov", n=n.pts, na.rm=TRUE): need at least 2 points to select a bandwidth automatically I got this error previously, but unfortunately could not get an answer from the list. I think it is about the memory. If it is about memory (which on our machine is 47.2 GiB), how can I solve this problem? Maybe parallel computing may do? Any help will be much appreciated. Best, Zeynep On Sat, Apr 5, 2014 at 1:38 PM, zeynep özkeserli <zeynep.ozkeserli@gmail.com> wrote: > Hi all (and if any chance, Dr. Aryee), > > I am working with 112 (56 532, 56 635) NimbleGen Promoter Medip arrays to > perform a CHARM analysis. > > First of all, I wanted to obtain the qc values and have a qcReport but I > get this error message: > > Error in plot.window(...) : need finite 'xlim' values > > My code is: > > dataDir<- getwd() > pd<- readDelim( files=pd$fileName, path=dataDir, sampleKey=pd) > rawData<- readCharm(files=pd$fileName, path=dataDir, sampleKey=pd) > qual<- qcReport(rawData, file="qcReport.pdf") > > R version: 3.03 > Memory: 47.2 GiB > Processor: Intel Xeon CPU E5645 @ 2.40GHz x 19 > OS type: 64-bit > Disk. 146.2 GB > > Has anyone have any idea about this? > > Thank you in advance, > > Best, > > Zeynep > [[alternative HTML version deleted]]
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