Question: Limma table results (time course analysis)
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gravatar for Gordon Smyth
5.6 years ago by
Gordon Smyth39k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth39k wrote:

NOTE: This is an answer to Limma table results (time course analysis)

Dear Stefania,

You have a replicated time course experiment with three time points. This is dealt with in detail in the limma User's Guide in Section 9.6.1 "Replicate time points".  You should be able to apply the advice in that section to your data without any problems.

The limma User's Guide tells you that spline curves are only for time course experiments with many time points.  You don't have many time points -- only three.  There is no advantage in using spline curves for a time course experiment with only three time points.  You cannot achieve anything that the simpler analysis presented in Section 9.6.1 would not achieve more explicitly.

Given that you have fitted spline curves, the test that you have conducted will correctly select genes whose change over time is different between the three genotypes.  However you cannot interpret the individual logFC columns in the toptable.  These are simply mathematically defined basis vectors for the spline curve -- they have no meaningful interpretation as individual columns.

Best wishes
Gordon

limma time course • 832 views
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