Dear Bioconductor users, I´m using the package EasyqpcR to analyze my qPCR data. Is there any way to extract the standard error calculation values which are given by the nrmData function and include them is a data frame for example? Also, if I include the slope calculation in a object ("efficiency") and use it in the nrmData function (....E=efficiency...) I get error. "Error in Ops.data.frame(E1, (ctmeanall1 - ctmean1)) : list of length 4 not meaningful" I solve the thing by creating a data frame: >efficiency_dataframe<-efficiency$Efficiency and use it as argument in the nrmData function. Is this the way to do? Thanks for your help! >sessionInfo() R version 3.0.2 (2013-09-25) Platform: x86_64-w64-mingw32/x64 (64-bit) locale: [1] LC_COLLATE=English_United Kingdom.1252 LC_CTYPE=English_United Kingdom.1252 [3] LC_MONETARY=English_United Kingdom.1252 LC_NUMERIC=C [5] LC_TIME=English_United Kingdom.1252 attached base packages: [1] graphics grDevices utils datasets stats methods base other attached packages: [1] ggplot2_1.0.0 EasyqpcR_1.5.0 loaded via a namespace (and not attached): [1] colorspace_1.2-4 digest_0.6.4 grid_3.0.2 gtable_0.1.2 [5] gWidgets_0.0-52 gWidgetsRGtk2_0.0-82 MASS_7.3-29 matrixStats_0.10.0 [9] munsell_0.4.2 plotrix_3.5-7 plyr_1.8.1 proto_0.3-10 [13] R.methodsS3_1.6.1 Rcpp_0.11.2 reshape2_1.4 RGtk2_2.20.29 [17] scales_0.2.4 stringr_0.6.2 tools_3.0.2 Tommaso -- Tommaso Raffaello, PhD University of Helsinki Department of Forest Sciences Latokartanonkaari 7 PO Box 27 00014 Helsinki (Finland) Phone number: +358 504486086 email: tommaso.raffaello@helsinki.fi email: tommaso.raffaello@gmail.com [[alternative HTML version deleted]]