Microarray data analysis for detecting alternative splicing
Last seen 18 months ago
I am interested in alternative splicing patterns for genes from this data set :
Any Bioconductor package for analyzing the data for alternative splicing?
Also the supplementary files has .bar extension files. I assume they are processed files, but they are binary. How can I view them?
Thanks for the help,
Last seen 1 hour ago
WEHI, Melbourne, Australia
The diffSplice function in the limma package detects differential splicing using exon-level expression values from exon microarrays or from RNA-seq.
Using a tiling array for the Saccharomyces cerevisiae genome is a little different, but you could perhaps group probes by putative genes and treat each probe as an exon.
Last seen 5.4 years ago
I am planning to use the diffsplice function. I could not figure out what sort of count data format does it accept. I am using featureCounts to generate the counts from BAM files.
Thanks heaps for your help!
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