Question: Interpretation of GeneGA results
gravatar for Maciej Jończyk
4.9 years ago by
Maciej Jończyk0 wrote:

Hi All,

I'm trying to optimize Streptococcus pyrogenes cas9 sequence (ca. 4000nt) for expression in maize.

GeneGA package seems optimal (command GeneGA, optimalizing both codon bias and mRNA secondary structure) but it has poor documentation. I've created custom CAI table. After ca. four days of analysis I've got sequence with approximately -1000 free energy, and plots shows rather sinusoidal trends than convergence.

Are here somebody who have used this package and could share knowledge about good program options for long RNAs? Vignette just states that "longer region needs larger popSize and iters".

Has anybody care about secondary structure? It seems that it is inevitable.

genega • 549 views
ADD COMMENTlink modified 4.9 years ago by tomas.bjorklund20 • written 4.9 years ago by Maciej Jończyk0
Answer: Interpretation of GeneGA results
gravatar for tomas.bjorklund
4.9 years ago by
tomas.bjorklund20 wrote:

Hi Maciej,

Can I ask you how you managed to specify maize as the organism? I cannot get any abbreviation to work but the default "ec"

I totally agree that the documentation for this package is really lacking. That is unfortunate, as there are no good alternatives in R or as command line tools, as I can see it.

ADD COMMENTlink written 4.9 years ago by tomas.bjorklund20
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