I have a couple of questions about estimation of dispersion in DeSeq2 vs. DeSeq. The paper and manual on DeSeq and DeSeq2 are very clear but I have a hard time understanding what is the ‘correct’ or ‘recommended’ method of estimating the dispersion is for RNA-Seq. Does this depend on the sample size? Are there other assumptions that have to be met for each dispersion estimate type (mean, local or parametric)? is there a particular reason behind ‘tag-wise’ estimation not being in DeSeq2?