I was trying to use topGO to find over-representation of some selected genes. I don't have probe ids, just the gene symbols for both the selected genes and the gene universe. For the topGO object, I have:
sampleGOdata <- new("topGOdata",
description = "Simple session", ontology = "BP",
allGenes = mygeneUniverse, geneSel = myselectedGenes,
nodeSize = 10,
annot = annFUN.db)
How can I create the annot object so that I can create my topGO object?