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Question: Question about the proper way to combine Illumina Human HT-12 v4 expression datasets from different labs
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gravatar for jaro.slamecka
3.8 years ago by
jaro.slamecka130
Mitchell Cancer Institute, Mobile AL, USA
jaro.slamecka130 wrote:

Hi,
I tried to study as much as I could about the matter but I'm still confused. I have an Illumina Human HT-12 v4 expression data from our lab's RNA samples. I would like to see if I can combine the dataset with one I downloaded from GEO, also an HT-12 v4, to see differentially expressed genes between our cells and theirs. I would like to ask, what is the correct workflow for this kind of analysis? Can the following be applied?

1. read Dataset1 with lumi, do vst, rsn
2. read Dataset2 with lumi, do vst, rsn
3. combine both using function "combine" based on common features
4. remove batch effect using ComBat
5. do the actual differential expression analysis in limma

Does the process cover all that is needed? Or does a more sophisticated method for combining need to be implemented? I'd like to have confidence in the analysis results.

Jaro

ADD COMMENTlink written 3.8 years ago by jaro.slamecka130

So, the differential expression would be between dataset1 and dataset2?  

ADD REPLYlink written 3.8 years ago by Sean Davis21k

Yes it would, the dataset1 has 3 samples and the dataset2 has several groups of 3 samples so the differential expression analysis would be done between the dataset1 and the individual groups from dataset2.

ADD REPLYlink written 3.8 years ago by jaro.slamecka130
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