Question: ddCt calculation using bioconductor package
0
4.7 years ago by
gv40
United States
gv40 wrote:

Hi Everyone,

I want to use ddCt package from Bioconductor . The instrument used for it is from Applied Biosystems ViiA7 Software v1.2 which gives out .eds file. How can I use to ddCt to analyse my files?

Any other package which can take .eds files??

qpcr ddct • 1.2k views
modified 4.5 years ago by Zhang, Jitao David110 • written 4.7 years ago by gv40
Answer: ddCt calculation using bioconductor package
0
4.5 years ago by
Switzerland
Zhang, Jitao David110 wrote:

Dear gv,

From version 1.24.0, it's possible to use a data.frame as input. Check 'example(InputFrame)' out.

Otherwise it's also possible to write a specific parser of eds files. Do you mind sharing an example with me? That can be quite helpful since I do not have access to such files.

If you like you can also write a parser by yourself, too. Check out the example of 'QuantStudioFrame', which is a function we recently added to support output files from LifeTechnologies QuantStudio software.

Best wishes,
David

Hi David,

Where can I find ddCT version 1.24.0, because I have just installed ddCt from bioconductor but the version I am getting is 1.22.0

> packageVersion("ddCt")
[1] ‘1.22.0’

I also have my qPCR data as .eds from QuantStudio 12k Flex and I very much want to analyze it using ddCT. So where can i find version 1.24.0

Thanks,

Stan