ddCt calculation using bioconductor package
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gv ▴ 40
@gv-6516
Last seen 11 weeks ago
United States

Hi Everyone,

I want to use ddCt package from Bioconductor . The instrument used for it is from Applied Biosystems ViiA7 Software v1.2 which gives out .eds file. How can I use to ddCt to analyse my files?

Any other package which can take .eds files??

Thanks in Advance

ddct qPCR • 1.8k views
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@zhang-jitao-david-5737
Last seen 2.7 years ago
Switzerland

Dear gv,

   From version 1.24.0, it's possible to use a data.frame as input. Check 'example(InputFrame)' out.

   Otherwise it's also possible to write a specific parser of eds files. Do you mind sharing an example with me? That can be quite helpful since I do not have access to such files. 

   If you like you can also write a parser by yourself, too. Check out the example of 'QuantStudioFrame', which is a function we recently added to support output files from LifeTechnologies QuantStudio software.

  Best wishes,
David

 

 

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Hi David,

Where can I find ddCT version 1.24.0, because I have just installed ddCt from bioconductor but the version I am getting is 1.22.0

> packageVersion("ddCt")
[1] ‘1.22.0’

I also have my qPCR data as .eds from QuantStudio 12k Flex and I very much want to analyze it using ddCT. So where can i find version 1.24.0

Thanks,

Stan

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Hi David,

Could you please provide the updated version of ddCt that supporting .eds file parsing function.

Best,

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