Help with cummeRbund
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mfahim • 0
@mfahim-7714
Last seen 7.2 years ago
Korea, Republic Of

I have biology background and am just starting to learn Rstudio. I have made the database from the cuffdiff output from cufflinks. But now I need to visualize the data and get to meaningful figures for publications.

I have installed cummeRbund but I have zilch programming skills and am unable to understand the commands.

I need someone to help me by writing full analysis script for the following samples.

I have three strains and one wildtype grown at two different temperatures 16C and 23C. I am looking for differentially expressed genes, isoforms, TSS..

I also need separate graphs for each isoform and their expression level (I know Sashimi in IGV is goo but it doesnot give me what I want i.e., separate quantification of each isoform).

What other analysis is possible? heatmaps.. etc.

 

Thanks heaps

cummeRbund Script analysis RNAseq cuffdiff • 1.2k views
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@andrewjskelton73-7074
Last seen 6 weeks ago
United Kingdom

You're literally asking other people to do the work for you? 

There's a user guide, which is pretty straight forward - http://www.bioconductor.org/packages/release/bioc/vignettes/cummeRbund/inst/doc/cummeRbund-manual.pdf

 

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b.nota ▴ 360
@bnota-7379
Last seen 2.0 years ago
Netherlands

Hi,

There is a Nature Protocols paper which describes the analysis with cummeRbund, http://www.nature.com/nprot/journal/v7/n3/full/nprot.2012.016.html

 

However if I see your experimental design, I would recommend edgeR for your analysis. It has a good manual, which is easy to understand for biologists.

Ben

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