I have several bigWig files from a ChIP-Seq expeiment. The files were generated in Galaxy using MACS and then converted from wig to bigwig using galaxy's Wig/BedGraph-to-bigWig converter.
When I attempt to import these bigwigs into R using the import.bw function I get the following error:
udc couldn't read 4 bytes from Galaxy215-[167_phage_input_Wig_BedGraph-to-bigWig].bigwig, did read 0
I suppose this is something rather stupid, but I'm not sure what may be the source of the problem or how to start troubleshooting this.
Does anyone has any idea what may cause this?
Thanks in advance
R version 3.1.1 (2014-07-10)
Platform: x86_64-pc-linux-gnu (64-bit)
attached base packages:
 stats4 parallel stats graphics grDevices utils datasets
 methods base
other attached packages:
 rtracklayer_1.26.2 GenomicRanges_1.18.4 GenomeInfoDb_1.2.4
 IRanges_2.0.1 S4Vectors_0.4.0 BiocGenerics_0.12.1
loaded via a namespace (and not attached):
 BBmisc_1.8 BatchJobs_1.5 BiocParallel_1.0.2
 Biostrings_2.34.1 DBI_0.3.1 GenomicAlignments_1.2.1
 RCurl_1.95-4.5 RSQLite_1.0.0 Rsamtools_1.18.3
 XML_3.98-1.1 XVector_0.6.0 base64enc_0.1-2
 bitops_1.0-6 brew_1.0-6 checkmate_1.5.1
 codetools_0.2-8 digest_0.6.8 fail_1.2
 foreach_1.4.2 iterators_1.0.7 sendmailR_1.2-1
 stringr_0.6.2 tools_3.1.1 zlibbioc_1.12.0