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Hello to everyone,
I have a question regarding vsn normalization.I have use rma to normalize raw data using affy package. When I tried using vsn normalization using vsn2 function in r to normalize my raw data, It displays this error.
"Error in MatrixFromNChannelSet(x, backgroundsubtract = backgroundsubtract, :
One or more elements of 'foreground' are not contained in 'channelNames(x)'."
I have a question whether vsn can be used to normalize the cel files of raw data?????
Could you please help me with this situation!!