I want to use the DiffBind software to compare chip-seq intensity between different cell conditions at all promoter regions. However, I noticed that a "score" column is needed in peak files to create a dba object at the beginning. Obviously, the artificial bed files with all promoters cannot have the score column. Please let me know how you import promoter regions in DiffBind.
I tried a bed file with the score column ''.", and got the following error message:
> samples<-dba(sampleSheet="samples.csv") MF01 J1 Reporter Negative 1 bed Error in Summary.factor(c(1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, : ‘max’ not meaningful for factors
Many thanks,
Xin