fold change in deseq
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tonja.r ▴ 80
@tonjar-7565
Last seen 7.4 years ago
United Kingdom

How do they estimate a fold change in the following situation (normalized counts already:

treated1 treated2 untreated1 untreated2
10           10           0.8             0.6 

or if all untreated groups have zero counts.

What kind of pseudo-counts is used?



 

deseq2 • 859 views
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@mikelove
Last seen 18 minutes ago
United States
Take a look at the DESeq2 paper for an intuitive description of the fold change estimate. It's not a pseudocount based method, but a Bayesian method, which uses the information from the experiment to determine the best estimate when there is little information (eg. low counts, which can vary a lot).
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