Question: deseq2 for TCGA tumor/normal paired sample from multiple patients
gravatar for luren
22 months ago by
United States
luren0 wrote:

Basically I want to run DEseq to analyze TCGA paired data. say in certain cancer type, there are 107 patients, 214 samples(paired). I used following code(pretty much default). By setting like this, I expect DEseq2 giving me a result using the pairing information as well as 107 patient as biological replicates, is this appropriate? I feel not confident because I use 107 different values for the factor-- patient.


sampleFiles <-c("1","2","3","4","5","6","7","8","9","10","11","12","13","14","15","16","17","18","19","20","21","22","23","24","25","26","27","28","29","30","31","32","33","34","35","36","37","38","39","40","41","42","43","44","45","46","47","48","49","50","51","52","53","54","55","56","57","58","59","60","61","62","63","64","65","66","67","68","69","70","71","72","73","74","75","76","77","78","79","80","81","82","83","84","85","86","87","88","89","90","91","92","93","94","95","96","97","98","99","100","101","102","103","104","105","106","107" "214")

#Above I use "1""2" to "214" as file name for 214 samples


#Above I use "1" "2"... to "107" as patient name for 107 patients


sampleTable<-data.frame(sampleName=sampleFiles, fileName=sampleFiles, condition=sampleCondition,patient=samplePatient)

ddsHTSeq<-DESeqDataSetFromHTSeqCount(sampleTable=sampleTable, directory=directory,design=~patient+condition)

colData(ddsHTSeq)$condition<-factor(colData(ddsHTSeq)$condition, levels=c("untreated","treated"))


write.table(res, file = 'myfile.txt',sep='\t')


ADD COMMENTlink modified 22 months ago by Michael Love13k • written 22 months ago by luren0

Just a tip to make your life easier. You could declare your sampleFiles and samplePatient variables like so:

sampleFiles <- as.character(1:214)
samplePatient <- as.character(rep(1:107, 2))


ADD REPLYlink modified 22 months ago • written 22 months ago by Steve Lianoglou12k

Many thanks for your tip!!!!

ADD REPLYlink written 22 months ago by luren0
gravatar for Michael Love
22 months ago by
Michael Love13k
United States
Michael Love13k wrote:
Yes that looks correct
ADD COMMENTlink written 22 months ago by Michael Love13k

Thanks a lot for fast response! But this gives me more than 10k out of 20k genes with p adj 10^-6 and lower. I am using TCGA BRCA data(214 total, from 107 patient, paired, raw counts.) Is this normal?Do you have any suggestions to shrunk it?


ADD REPLYlink written 22 months ago by luren0
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