Hi All! I have tried to process 24 Affymetrix HGU133plus2 chips. I can load them, make RMA, but all other normalisation methods fail (vsn, dchip, mas5), with the message: "cannot allocate a vector of size 243457 kb". In have tried to set the memory limit to 2048 and to 3000000000. (I have 2Gb RAM in a dual xeon computer, so the hardware should not be the problem.) Is there a way to solve this problem? Thank you for your help. Balazs ===== ------------------------- Balazs Gyorffy MD, PhD Semmelweis University, 2nd Dept. of Internal Medicine Szentkiralyi u. 46, H-1088 Budapest, Hungary tel: 003612660926 / 5622 fax: 003612660926 / 5596 email: zsalab2@yahoo.com

Hi Balazs, can you provide more details: operating system, R version? Best regards Wolfgang Dr_Gyorffy_Balazs wrote: > Hi All! > > I have tried to process 24 Affymetrix HGU133plus2 chips. I > can load them, make RMA, but all other normalisation > methods fail (vsn, dchip, mas5), with the message: "cannot > allocate a vector of size 243457 kb". In have tried to set > the memory limit to 2048 and to 3000000000. (I have 2Gb RAM > in a dual xeon computer, so the hardware should not be the > problem.) > > Is there a way to solve this problem? > > Thank you for your help. > Balazs > > ===== > ------------------------- > Balazs Gyorffy MD, PhD > Semmelweis University, 2nd Dept. of Internal Medicine > Szentkiralyi u. 46, H-1088 Budapest, Hungary > tel: 003612660926 / 5622 > fax: 003612660926 / 5596 > email: zsalab2@yahoo.com > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor -- Best regards Wolfgang ------------------------------------- Wolfgang Huber European Bioinformatics Institute European Molecular Biology Laboratory Cambridge CB10 1SD England Phone: +44 1223 494642 Fax: +44 1223 494486 Http: www.ebi.ac.uk/huber