When I try to call the function getZSCores on my 4C data (mouse) I got the following error:
> data_fcf <- getZScores(data_fc,minCount=10,fitFun=distFitMonotone)
gene-wise dispersion estimates
NOTE: fitType='parametric', but the dispersion trend was not well captured by the
function: y = a/x + b, and a local regression fit was automatically substituted.
specify fitType='local' or 'mean' to avoid this message next time.
final dispersion estimates
Error in getZScores(data_fc, minCount = 10, fitFun = distFitMonotone) :
Failed to estimate the parameters of the Variance stabilizing transformation.
Looking at the code it seems due to the fact that parametric fit did not work so he stopped after that.
Is it not possible to go further in the analysis with a local fit?