I am using FourCseq with one 4C experiment with 2 biological replicates and 2 view points.:
DataFrame with 4 rows and 5 columns
viewpoint condition replicate bamFile sequencingPrimer
<character> <factor> <numeric> <character> <character>
1 FLC1 A 1 INDEX_1_3.sorted.bam first
2 FLC1 A 2 INDEX_1_4.sorted.bam first
3 FLC2 B 1 INDEX_1_5.sorted.bam first
4 FLC2 B 2 INDEX_1_6.sorted.bam first
When I run:
fcf <- getZScores(fc)
I get the following error:
Error in getZScores(fc) :
None or more than one viewpoint are contained in the 'FourC' object.
Use a 'FourC' object that contains only one viewpoint.
The pipeline doesnt support multiple view points?
Could you please help me with this if it is possible to run it with 2 viewpoints ?