HTQPCR - create qPCR set from a matrix of CT values
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@sharvari-gujja-6614
Last seen 1 hour ago
United States

Hello,

I am trying to create a qPCR set using the R package HTqPCR. My input file is a matrix of CT values for 254 miRNAs from 27 samples ( divided in 3 groups).

How do I use theĀ readCtData function to create qPCR set for my input file?

Thanks

Sharvari

HTqPCR • 871 views
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@gordon-smyth
Last seen 7 hours ago
WEHI, Melbourne, Australia

Alternatively you could input the matrix of Ct values directly into limma, then there's no need for a qPCR set. See for example Performing differential gene analysis on RT PCR experimental data

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