Question: Manually compute NUSE score
1
3.9 years ago by
jacorvar40
European Union
jacorvar40 wrote:

Hi,

I would like to manually compute NUSE scores for Affy Microarray Data, but I do not know to do it. I computed the RLE score by subtracting its median to a matrix of intensities, but can't find a formula for NUSE.

modified 3.9 years ago by Diego Diez750 • written 3.9 years ago by jacorvar40
0
3.9 years ago by
Diego Diez750
Japan
Diego Diez750 wrote:

Why not use the affyPLM package? You can find in the package's web site Ben Bolstad's publications where it is descibed. It is also described in this paper by Rafael Irizarry: Assessing affymetrix GeneChip microarray quality.

1

Or you could use the oligo package as well.