Question: Manually compute NUSE score
1
gravatar for jacorvar
3.9 years ago by
jacorvar40
European Union
jacorvar40 wrote:

Hi,

I would like to manually compute NUSE scores for Affy Microarray Data, but I do not know to do it. I computed the RLE score by subtracting its median to a matrix of intensities, but can't find a formula for NUSE.

 

ADD COMMENTlink modified 3.9 years ago by Diego Diez750 • written 3.9 years ago by jacorvar40
Answer: Manually compute NUSE score
0
gravatar for Diego Diez
3.9 years ago by
Diego Diez750
Japan
Diego Diez750 wrote:

Why not use the affyPLM package? You can find in the package's web site Ben Bolstad's publications where it is descibed. It is also described in this paper by Rafael Irizarry: Assessing affymetrix GeneChip microarray quality.

ADD COMMENTlink written 3.9 years ago by Diego Diez750
1

Or you could use the oligo package as well.

ADD REPLYlink written 3.9 years ago by James W. MacDonald51k
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