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green.rebeccam
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@greenrebeccam-9713
Last seen 8.2 years ago
Hi All, I am working with an RNAseq data set of 20 samples across 4 geneotypes with increasing severity of the phenotype across the genotypes. One of the questions we are trying to answer is does the variance of gene expression (level of disregulation) change across the groups. I know that DeSeq2 calculates a gene-wise diserpersion estimate. Does anyone have any ideas to use this to calculate a sample dispersion level and to compare that to other samples? - Thanks!!!!