Hi,

When reading my data into HTqPCR using readCtData I get an error. I'd be grateful for any pointers as to what I am doing wrong. My data files are all saved as .txt files in SDS format. I have not changed anything from the SDS output other than changing the file type, so I believe the default column locations should correspond. Having started by specifying my file location, using read.delim as per instructions, when I come to read in the data, this happens:

raw <- readCtData(files = files$File, path = NULL, format="SDS", n.features = 96, n.data = 4, samples, na.value = 40)

Error in readLines(con = readfile, n = 100) : 'con' is not a connection

sessionInfo()

R version 3.2.3 (2015-12-10)

Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 7 x64 (build 7601) Service Pack 1

locale:
[1] LC_COLLATE=English_United Kingdom.1252  LC_CTYPE=English_United Kingdom.1252    LC_MONETARY=English_United Kingdom.1252 LC_NUMERIC=C                           
[5] LC_TIME=English_United Kingdom.1252    

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] HTqPCR_1.24.0       limma_3.26.8        RColorBrewer_1.1-2  Biobase_2.30.0      BiocGenerics_0.16.1

loaded via a namespace (and not attached):
 [1] gtools_3.5.0          bitops_1.0-6          affy_1.48.0           stats4_3.2.3          KernSmooth_2.23-15    BiocInstaller_1.20.1  gplots_3.0.1          zlibbioc_1.16.0      
 [9] gdata_2.17.0          affyio_1.40.0         preprocessCore_1.32.0 tools_3.2.3           caTools_1.17.1   

Any help appreciated!

Thanks,

Liz