When I type the following code:
> x = processAmplicons("Index2.Plate10.fastq", barcodefile="Samples1.txt",
+ hairpinfile="Hairpins sense.txt", hairpinStart=29, hairpinEnd=50, verbose=TRUE)
Error in processAmplicons("Index2.Plate10.fastq", barcodefile = "Samples1.txt", :
Hairpin sequence length is set to 22, there are hairpin sequences not with specified length.
I checked the length of my hairpin sequences, in my hairpin file/reference library and they are all 22nt long.
I don't understand what is going wrong. If you have any ideas of what I can do/check please let me know.