Hello, I recently installed the R (version 2.0.1) and Bioconductor on my computer. I installed the packages in "affy", the affyGui and the limmaGui on my computer and they all appear in the Load Package window. After trying to load the affyGui or the limmaGui packages I receive the following message entitled: "Tcl/Tk Extension(s) Not Found"- limmaGUI requires the Tcl/Tk extensions, BWidget and Tktable. You must have Tcl/Tk installed on your computer, not just the minimal Tcl/Tk installation which comes with R (for Windows). If you do have Tcl/Tk installed, including the extensions (e.g. using the ActiveTcl distribution in Windows), make sure that R can find the path to the Tcl library, e.g. C:\Tcl\lib (on Windows) or /usr/lib (on Linux/Unix) or /sw/lib on Mac OSX. If you don't know how to set environment variables in Windows, one way to make sure that R can find the Tcl/Tk extensions Tktable2.8 and bwidget1.6 is to copy them from your ActiveTcl installation e.g. in C:\Tcl\lib into the Tcl subdirectory of your R installation. If you do understand how to set environment variables... make sure that you have the TCL_LIBRARY environment variable set to the appropriate path, e.g.C:\Tcl\lib\tcl8.4 and the MY_TCLTK environment variable set to a non-empty string, e.g. "Yes". If using Windows, be sure to read the R for windows FAQ at http://www.stats.ox.ac.uk/pub/R/rw-FAQ.html If your Tcl/Tk extensions still can't be found, try addTclPath(""). This could be put in $HOME/.Rprofile If you need further instructions, please contact your system administrator and consider emailing r-help@stat.math.ethz.ch, or browse through the R-help archives for a similar question. The URLs for Tktable and BWidget are: http://tktable.sourceforge.net http://tcllib.sourceforge.net I searched the mailing list for this specific problem and did not find any answer. I also serached the PDF "R for Windows Users" but I could not understand the things written about Tcl/Tk. How can I fix this problem. Thank you, Drigues Noam The Eve Topf and USA National Parkinson Foundation Centers of Excellence for Neurodegenerative diseases Laboratory of Prof. Youdim M.B.H. Department of Pharmacology The Bruce Rappaport Faculty of Medicine Technion- Israel Institute of Technology P.O.B 9649, Haifa 31096, Israel E-mail: drigues@tx.technion.ac.il Tel (W): +972-4-8295289 Tel (H): +972-4-8522346 Tel (M): +972-54-4910902 Fax (W): +972-4-8513145