I am trying to figure out how the ExpressionSet is working. I am new to R and have some problems.
I have a lot of data and want to normalize it with the CRMN package. My data consists of samples and analytes. Some of these analytes are Internal Standards. So with the ExpressionSet I want to provide the data to the normalization functions from the CRMN package.
So I have the samples and the analytes but how do I define which analyt is an Internal Standard? Thats what I have so far:
exprsFile <- "C:/Users/..../exprsData.txt"
exprs <- as.matrix(read.table(exprsFile, header=TRUE, sep=",",row.names=1,as.is=TRUE))
minimalSet <- ExpressionSet(assayData=exprs)
Isn't there possibility to use the sample in .rda format from the CRMN package and just overwrite it with my data?