Cannot load old SummarizedExperiment in R 3.3, Bioc 3.3
1
0
Entering edit mode
@ryan-c-thompson-5618
Last seen 8 months ago
Scripps Research, La Jolla, CA

I have some SummarizedExperiment objects from previous Bioconductor and R versions, and I've been using them in subsequent analyses by running updateObject() on them. However, after updating to R 3.3, I now get the following when I try to do so:

> sexp <- readRDS("promoter-group-counts-1kb.RDS") %>% updateObject
Error in validObject(result) :
  invalid class "SummarizedExperiment" object:
  nb of rows in 'assay' (35631) must equal nb of rows in 'rowData' (0)
In addition: Warning message:
In updateObjectFromSlots(object, ..., verbose = verbose) :
  dropping slot(s) 'rowData', 'exptData' from object = 'SummarizedExperiment'

I don't remember exactly what version of Bioc these were generated under, but I know it was before the introduction of RangedSummarizedExperiment. In any case, you can access the file here: https://www.dropbox.com/s/5d3vktytsb0b22x/promoter-group-counts-1kb.RDS?dl=0

Can anyone help me figure out how to load this data into the new BioC version?

summarizedexperiment backwards compatibility • 1.1k views
ADD COMMENT
2
Entering edit mode
@herve-pages-1542
Last seen 1 day ago
Seattle, WA, United States

Hi Ryan,

The "updateObject" method for SummarizedExperiment was removed in BioC 3.3. It seems that it was a little bit too early for that, sorry. Note that updateObject() is typically a one-time use i.e. one should normally re-serialize the object after running updateObject() on it. That way all the old serialized SummarizedExperiment objects hanging around get replaced by new ones little by little.

I restored the "updateObject" method in SummarizedExperiment 1.2.3 (release) and 1.3.4 (devel). Please use it to fix and re-serialize your old SummarizedExperiment objects.

Thanks,

H.

ADD COMMENT
0
Entering edit mode

Thanks. Good to know. I'll update all my SummarizedExperiment objects and re-serialize them.

ADD REPLY

Login before adding your answer.

Traffic: 704 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6