Dear EdgeR users
I am working on a RNASeq dataset with extremely small sample size (2 samples in each group; N=4) and I am trying to find any DEG's using these samples (I am aware of the consequences of using very small sample size). I did find some DEG's with P.values as low as 6.9e-17 and an equivalent FDR of 8.14e-13. Although it seems encouraging to see some differences in my samples, I am more concerned about so low P.values with such a small sample size (This is because of my limited statistical knowledge). Just wondering how EdgeR calculates P.values with such low sample sizes. Any comments or opinions are greatly appreciated. Many thanks for your help.