Hi, they finally updated the R on our cluster so I am now using DESeq2_1.10.1 with R 3.2.2.. However, I was surprised to see that the option "fast" in rlog was not available anymore, is there a reason why? Was the approximation not good/correct? I tried to run rlog in v1.10.1 but it's taking ages (3 days and it's not done). I have 683 samples and I know that, in the vignette, it is written that with more than 100 samples it is better to use the vst function. However, the library sizes are very different so I think that rlog would be a better choice in my case. If the approximation was correct I am thinking of taking the raw code from the old package and run just rlog with that on my dataset. Do you have other suggestions?