Normalizing RNA-Seq data without replicates.
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@humberto_munoz-10903
Last seen 6.1 years ago

I would like to normalize some RNA-Seq samples without replicates using different techniques than RPKM. Thanks for any suggestions. 

Humberto

normalization • 648 views
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You mean you have only one sample?  Or you mean you have several samples, none of which has a replicate?

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I have 11samples without replicates. Each sample is for different condition. 

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chris86 ▴ 400
@chris86-8408
Last seen 2.7 years ago
UCL, United Kingdom

TPM (transcripts per million) is better for comparing expression strength.

https://haroldpimentel.wordpress.com/2014/05/08/what-the-fpkm-a-review-rna-seq-expression-units/

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