Question

Normalizing RNA-Seq data without replicates.

0

Entering edit mode

humberto_munoz • 0

@humberto_munoz-10903

Last seen 7.8 years ago

I would like to normalize some RNA-Seq samples without replicates using different techniques than RPKM. Thanks for any suggestions.

Humberto

normalization • 965 views

ADD COMMENT • link updated 7.8 years ago by chris86 ▴ 420 • written 7.8 years ago by humberto_munoz • 0

0

Entering edit mode

You mean you have only one sample? Or you mean you have several samples, none of which has a replicate?

ADD REPLY • link 7.8 years ago Sean Davis 21k

0

Entering edit mode

I have 11samples without replicates. Each sample is for different condition.

ADD REPLY • link 7.8 years ago humberto_munoz • 0

score 0 · Answer 1 · 2016-06-20

0

Entering edit mode

chris86 ▴ 420

@chris86-8408

Last seen 4.4 years ago

UCL, United Kingdom

TPM (transcripts per million) is better for comparing expression strength.

ADD COMMENT • link 7.8 years ago chris86 ▴ 420