I've been using Rsubread with a range of new and old FASTQ files and it has been working fine. The latest batch seems to be having some problems and for some of the files I get the following error after it crashes
1: .C("R_align_wrapper", as.integer(n), as.character(cmd), PACKAGE = "Rsubread")
2: align(index = "reference_index", minFragLength = 35, nthreads = 30, readfile1 = "RS-02239140_N3_DMSO_GFP_R1.fastq.gz", output_file = "RWPE.GFP.DMSO.3.BAM")
I think i saw an earlier post about 10 months ago with a similar issue but couldn't see a resolution.
I'm using Rsubread_1.22.2
Anyone got any thoughts?