Using pathview with uncommon species leads to Errors
1
0
Entering edit mode
rafre • 0
@rafre-11151
Last seen 7.8 years ago

Hello together,

I want to plot my RNA-Seq data to the Corynebacterium glutamicum ATCC13032 (Bielefeld) KEGG pathway, but unfortunatly this is not working wiht my organism. I found an example for B. subtilis which is working pretty fine, but changing the species name ( i looked it up in the data(korg)) leads to an error.

library(pathview)
> bsu.dat.kegg <- sim.mol.data(mol.type="gene"
+                              ,id.type="kegg"
+                              ,species="bsu"
+                              ,nmol=3000)
> 
>     pv.out.bs <- pathview(gene.data = bsu.dat.kegg
+                        , gene.idtype="kegg"
+                        , pathway.id = "00010"
+                        , species = "bsu"
+                        , out.suffix = "bsu.kegg")
Info: Working in directory C:/Users/....
Info: Writing image file bsu00010.bsu.kegg.png
> cgb.dat.kegg <- sim.mol.data(mol.type="gene"
+                              ,id.type="kegg"
+                              ,species="cgb"
+                              ,nmol=3000)
Error in .getUrl(url, .listParser, nameColumn = 1, valueColumn = 2) : 
  Bad Request (HTTP 400).
> traceback()
5: stop(http_condition(x, "error", task = task, call = call))
4: stop_for_status(response)
3: .getUrl(url, .listParser, nameColumn = 1, valueColumn = 2)
2: keggConv("ncbi-geneid", species)
1: sim.mol.data(mol.type = "gene", id.type = "kegg", species = "cgb", 
       nmol = 3000)
> sessionInfo()
R version 3.3.1 (2016-06-21)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 7 x64 (build 7601) Service Pack 1

locale:
[1] LC_COLLATE=German_Germany.1252  LC_CTYPE=German_Germany.1252    LC_MONETARY=German_Germany.1252
[4] LC_NUMERIC=C                    LC_TIME=German_Germany.1252    

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] pathview_1.12.0      org.Hs.eg.db_3.3.0   AnnotationDbi_1.34.4 IRanges_2.6.1        S4Vectors_0.10.2    
[6] Biobase_2.32.0       BiocGenerics_0.18.0 

loaded via a namespace (and not attached):
 [1] KEGGgraph_1.30.0  XML_3.98-1.4      Biostrings_2.40.2 png_0.1-7         R6_2.1.2          grid_3.3.1       
 [7] DBI_0.4-1         RSQLite_1.0.0     httr_1.2.1        graph_1.50.0      zlibbioc_1.18.0   curl_0.9.7       
[13] XVector_0.12.0    Rgraphviz_2.16.0  tools_3.3.1       KEGGREST_1.12.2  

Somehow the download of KEGGpathways is possible with the species ID.

download.keggpathway.id = "01100"
              , species = "cgb"
              , kegg.dir = myDir
              , file.type=c("xml", "png"))

I hope you can reproduce my Error or show me a way out

Thanks in advance

pathview Kegg rnaseq data visualization • 1.9k views
ADD COMMENT
1
Entering edit mode
Luo Weijun ★ 1.6k
@luo-weijun-1783
Last seen 10 months ago
United States
It appears to be a KEGG REST API problem. I noticed that KEGG REST API had some issue lately as shown in the build error of the package: http://bioconductor.org/checkResults/release/bioc-LATEST/KEGGREST/ You can check this again when the build error is gone.
ADD COMMENT

Login before adding your answer.

Traffic: 723 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6