backgroundCorrect(method="normexp") problems
1
0
Entering edit mode
@palmer-lance-1222
Last seen 9.6 years ago
I am just starting to do microarray analysis. I have been trying to normalize 4 chips (two dye-swap experiments done in biological replicates) After doing backgroundCorrect(method="normexp") , one of the arrays has all of its $G values at NaN. This does not happen when any other method is used. Can someone tell me why this is, and if this can be fixed? Thanks Lance Palmer
Microarray Microarray • 674 views
ADD COMMENT
0
Entering edit mode
@gordon-smyth
Last seen 9 hours ago
WEHI, Melbourne, Australia
You'll see this question asked a few times in the mailing list archives. Try upgrading your version of the limma package. Gordon >Date: Thu, 28 Apr 2005 16:44:41 -0400 >From: "Palmer, Lance" <palmer@cshl.edu> >Subject: [BioC] backgroundCorrect(method="normexp") problems >To: <bioconductor@stat.math.ethz.ch> > >I am just starting to do microarray analysis. I have been trying to >normalize 4 chips (two dye-swap experiments done in biological replicates) > >After doing backgroundCorrect(method="normexp") , one of the arrays has >all of its $G values at NaN. > >This does not happen when any other method is used. > >Can someone tell me why this is, and if this can be fixed? > >Thanks >Lance Palmer
ADD COMMENT

Login before adding your answer.

Traffic: 940 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6