Hello I am new to R and am trying to read Agilent microarray files using read.Agilent in the marray library. Please see below for the R version installed on my computer platform i686-pc-linux-gnu arch i686 os linux-gnu system i686, linux-gnu status major 2 minor 0.0 year 2004 month 10 day 04 language R Please see cammand line arguments below: data.dir is the directory path > hcc.targets <- read.marrayInfo(file.path(data.dir, "target.text")) > hcc.layout <- read.marrayLayout(fname=file.path(data.dir, "layout.text"), ngr=1, ngc=1, nsr=105, nsc=215, ctl.col=6, skip=0) > > hcc.gnames <- read.marrayInfo(file.path(data.dir, "layout.text"), info_id=4:5, labels=4, skip=10) > > fnames <- dir(path=data.dir, pattern=paste("*", ".txt", sep="\.")) > >hcc <- read.Agilent(fnames, path="data.dir", name.Gf="gMedianSignal", name.Gb="gBGMedianSignal", name.Rf = "rMedianSignal", name.Rb = "rBGMedianSignal", layout=hcc.layout, gnames=hcc.gnames, targets=hcc.targets, skip=10, sep="\t", quote="\"", DEBUG=TRUE) However I am getting an error: Error in read.Agilent(fnames, path = "data.dir", name.Gf = "gMedianSignal" , Object "descript" not found I have checked the file and there doesn't appear to be any problems opening it and there are no strange characters. I am not sure what I am doing wrong or what to try next. Can you please help? Many thanks in advance. Archana Sharma-Oates