Question: Meta-analysis on rna-seq data without replicates
gravatar for bioinformatics
3.1 years ago by
bioinformatics10 wrote:

Hello ! 


I have four data-sets. These data-sets are from different studies and or cell strains.

Two of them do only have a control and a treated vector.


I was following the default approach for the meta-analysis for rna-seq :


I understand that i have to perform the same filtering/approach on all data-sets in order to compare them properly. In order to do this it is essential to have uniform data-sets. This is most likely not the case for two of the data-sets so i will  have to find a way to make them uniform.

In the tutorial HTSFilter is used , but it can not be performed on data-sets with no replicates. My problem is that i really want to use all the data-sets.

What is the ( most likely ) best way to get to do this ?

I am very grateful for any help ! 



ADD COMMENTlink modified 3.1 years ago by Michael Love25k • written 3.1 years ago by bioinformatics10


If you want to ask the authors of metaRNASeq a question about the recommended approach to using their software, you should add their software as a tag. I've added this and removed DESeq2.

ADD REPLYlink written 3.1 years ago by Michael Love25k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 212 users visited in the last hour