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Question: FPKM normalization questions!
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gravatar for fromhj304
20 months ago by
fromhj30410
fromhj30410 wrote:

Hi all, I'm having a hard time understanding a concept in bioinformatics. So right now, I'm learning FPKM values. I'm not sure why we need this value in the first place. What is importance and what does it tell you that raw count data don't?

Also, why do we need to normalized the data? I don't quite understand the point of normalization. What is the difference between normalized data and un-normalized data?

 

Thank you so much!

ADD COMMENTlink modified 20 months ago by Aaron Lun19k • written 20 months ago by fromhj30410
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gravatar for Aaron Lun
20 months ago by
Aaron Lun19k
Cambridge, United Kingdom
Aaron Lun19k wrote:

Perhaps you should do some reading of the RNA-seq normalization literature:

There's probably more that I haven't listed, but a quick Google should fill the gaps.

ADD COMMENTlink written 20 months ago by Aaron Lun19k
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