Question: FPKM normalization questions!
gravatar for fromhj304
2.2 years ago by
fromhj30410 wrote:

Hi all, I'm having a hard time understanding a concept in bioinformatics. So right now, I'm learning FPKM values. I'm not sure why we need this value in the first place. What is importance and what does it tell you that raw count data don't?

Also, why do we need to normalized the data? I don't quite understand the point of normalization. What is the difference between normalized data and un-normalized data?


Thank you so much!

ADD COMMENTlink modified 2.2 years ago by Aaron Lun21k • written 2.2 years ago by fromhj30410
gravatar for Aaron Lun
2.2 years ago by
Aaron Lun21k
Cambridge, United Kingdom
Aaron Lun21k wrote:

Perhaps you should do some reading of the RNA-seq normalization literature:

There's probably more that I haven't listed, but a quick Google should fill the gaps.

ADD COMMENTlink written 2.2 years ago by Aaron Lun21k
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