Question: i need to have a package for reads alignment to reference genome
0
gravatar for hassangiahi
3.0 years ago by
hassangiahi0 wrote:

hi..

i need help so much to sole my problem .

i have 4 reds library and one reference genome .after the quality assessment and trimming of the reads i need to aligned the reads to a refrence genome . but i do not find any package for wnidows 7 ( my windows).

my reads format is FASTQ and i want to alignment their to a reference genome....what i doing???

rnaseq • 484 views
ADD COMMENTlink modified 3.0 years ago by Hotz, Hans-Rudolf400 • written 3.0 years ago by hassangiahi0

No offense, but I think you need more support than people online on a sporadic base can offer. Do you have someone in your institute with more experience with regard to this type of analysis?

ADD REPLYlink written 3.0 years ago by WouterDeCoster110
Answer: i need to have a package for reads alignment to reference genome
2
gravatar for chris86
3.0 years ago by
chris86380
UCL, United Kingdom
chris86380 wrote:

almost exclusively people use linux or a mac not windows7 for bioinformatics. you probably want to find a bioinformatican. if you want to do it you'll need linux and a tool called tophat and depending on your library size, many cpu cores/ a cluster. if your good at computers you could do it, but it will take a long time to learn everything.

ADD COMMENTlink modified 3.0 years ago • written 3.0 years ago by chris86380

tanks sooo much chirsss

ADD REPLYlink written 3.0 years ago by hassangiahi0
Answer: i need to have a package for reads alignment to reference genome
1
gravatar for Hotz, Hans-Rudolf
3.0 years ago by
Switzerland
Hotz, Hans-Rudolf400 wrote:

Have a look at the QuasR package...this should work on windows.

Regards, Hans-Rudolf

 

 

 

ADD COMMENTlink written 3.0 years ago by Hotz, Hans-Rudolf400

oh yes ....

but this version of QuasR is not compatible whit my windows . since my windows is 32 bit and the QuasR is compatible with 64 bit .... 

im very bad luck!!!!!!1

tanle you for your answer!!!!!

ADD REPLYlink written 3.0 years ago by hassangiahi0
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