Instead of counts (non-negative integers) required by DeSeq2, I have negative numbers and fractions,
and I need to use DeSeq's statistics to find out significantly up/down genes.
Since for my data, min=-10 and max=10, I can convert it into counts by
- first multiplying with a constant eg. 1000 to preserve the decimal precision.
- and then add some constant value eg. 10000 to make everything positive.
Would it hamper my analysis?