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Question: Return features from a single strand using vmatchPattern
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gravatar for jma1991
12 months ago by
jma199130
jma199130 wrote:

I am searching the genome for occurrences of a very small motif:

matches <- vmatchPattern(pattern = "TCAG", subject = BSgenome.Mmusculus.UCSC.mm10, exclude = c("M", "_"))

Because the motif is it's own reverse complement the vmatchPattern function returns matches on the positive and negative strand:

GRanges object with 13339240 ranges and 0 metadata columns:
             seqnames               ranges strand
                <Rle>            <IRanges>  <Rle>
         [1]     chr1   [3000191, 3000194]      +
         [2]     chr1   [3000813, 3000816]      +
         [3]     chr1   [3001048, 3001051]      +
         [4]     chr1   [3001119, 3001122]      +
         [5]     chr1   [3001795, 3001798]      +
         ...      ...                  ...    ...
  [13339236]     chrY [90843570, 90843573]      -
  [13339237]     chrY [90844087, 90844090]      -
  [13339238]     chrY [90844334, 90844337]      -
  [13339239]     chrY [90844496, 90844499]      -
  [13339240]     chrY [90844695, 90844698]      -

I would like instead for it to return all matches on either the positive or negative strand. From there I can then just reset the strand information to ambiguous:

strand(matches) <- "*"

Is there an option within vmatchPattern to return matches from a single strand? Similar to matchPattern which returns the result with ambiguous strand information. Granted, I could just filter out all minus or plus strand ranges:

matches <- matches[strand(matches) == "+"]

I just wondered if there was a way within the vmatchPattern function itself which I was missing?

 

ADD COMMENTlink modified 12 months ago by Hervé Pagès ♦♦ 13k • written 12 months ago by jma199130
0
gravatar for Hervé Pagès
12 months ago by
Hervé Pagès ♦♦ 13k
United States
Hervé Pagès ♦♦ 13k wrote:

Hi,

Note that TCAG is not a palindrome:

> motif <- DNAString("TCAG")
> reverseComplement(motif)
  4-letter "DNAString" instance
seq: CTGA

 

Anyway, to answer your question: no, vmatchPattern() doesn't have an option for searching only one strand of the genome. Filtering out the matches that belong to a given strand like you did is probably the best/easiest  way to work around this.

Cheers,

H.

ADD COMMENTlink written 12 months ago by Hervé Pagès ♦♦ 13k
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