I am interested in analyzing transcription factor (TF) activity using altered gene expression of target genes.
Can we predict/quantify transcription factor activation using microarray gene expression data? Is there any bioconductor pacakges to do that?
I am planning to collect the target gene list of TF and sum up whether they are differentially expressed as an indicator of TF activity. But the approach sounds too simple and I am trying to find out whether there are other methods or tools available. Any suggestions or relevant papers/pointer will be very helpful.
Thank you for your time