Hello 

I am trying to DEG by Limma with R i got error message , can any one help me

> fit = lmFit(celfiles.gcrma, design)
Error in lmFit(celfiles.gcrma, design) : 
  row dimension of design doesn't match column dimension of data object

Well, the error message seems pretty self explanatory. There has to be a column of the data object 'celfiles.gcrma' for each RNA sample you are trying to analyze and a row of the design matrix for each sample. But the number of columns of 'celfiles.gcrma' doesn't agree with the number of rows of 'design'. So over to you -- time for you to check what you've done so far.

Try typing dim(celfiles.gcrma) and dim(design).