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Question: Row and column design for Micro-array data data analysis?
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gravatar for mathavanbioinfo
11 months ago by
mathavanbioinfo0 wrote:

Hello 

I am trying to DEG by Limma with R i got error message , can any one help me

> fit = lmFit(celfiles.gcrma, design)
Error in lmFit(celfiles.gcrma, design) : 
  row dimension of design doesn't match column dimension of data object

 

ADD COMMENTlink modified 11 months ago by Gordon Smyth32k • written 11 months ago by mathavanbioinfo0
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gravatar for Gordon Smyth
11 months ago by
Gordon Smyth32k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth32k wrote:

Well, the error message seems pretty self explanatory. There has to be a column of the data object 'celfiles.gcrma' for each RNA sample you are trying to analyze and a row of the design matrix for each sample. But the number of columns of 'celfiles.gcrma' doesn't agree with the number of rows of 'design'. So over to you -- time for you to check what you've done so far.

Try typing dim(celfiles.gcrma) and dim(design).

ADD COMMENTlink modified 11 months ago • written 11 months ago by Gordon Smyth32k
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